Despite much research, our understanding of the rules by which cis-regulatory sequences are translated into expression levels is still lacking. We devised a method for obtaining parallel and highly accurate expression measurements of thousands of fully designed promoters, and applied it to measure the effect of systematic changes to location, number, orientation, affinity and organization of transcription factor (TF) binding sites and of nucleosome disfavoring sequences. Our analyses reveal a clear relationship between expression and binding site number, and TF-specific dependencies of expression on the distance between sites and gene starts including a striking ~10bp periodic relationship. We also demonstrate the utility of our approach for measuring TF sequence specificities and sensitivity of TF sites to surrounding sequence context, and for profiling the activity of most yeast transcription factors. Our method is readily applicable for studying both the cis and trans effects of genotype on transcriptional, post-transcriptional, and translational control.
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机译:用于植物转化的重组多核苷酸序列包括基因生物合成tms1 AUX CONTROL KLUBNESPETSIFICHNOGO-B33 patatin基因启动子CLASS I马铃薯(Solanum tuberosum L.),植物细胞,转基因植物及其包含基因B33 DESIGN:tms1的后代,以及方法器官特异性调节活性生长素