Insect Ryanodine Receptor: Distinct But Coupled Insecticide Binding Sites for N-C3H3Chlorantraniliprole Flubendiamide and 3HRyanodine

摘要

Radiolabeled anthranilic diamide insecticide [N-C³H3]chlorantraniliprole was synthesized at high specific activity and compared with phthalic diamide insecticide flubendiamide and [³H]ryanodine in radioligand binding studies with house fly muscle membranes to provide the first direct evidence with a native insect ryanodine receptor that the major anthranilic and phthalic diamide insecticides bind at different allosterically coupled sites, i.e. there are three distinct Ca²⁺-release channel targets for insecticide action.