A Fiberoptic (Photodynamic Therapy Type) Device with a Photosensitizer and Singlet Oxygen Delivery Probe Tip for Ovarian Cancer Cell Killing

摘要

A portable “fiber optic-based sensitizer delivery” device has been developed and studied. Before there might be success in photodynamic therapy (PDT) and anti-bacterial ambitions, an understanding of basic factors on device performance were needed. Thus, the device was examined for the localized delivery of sensitizer molecules in ovarian cancer cells and production of high concentrations of singlet oxygen for their eradication in vitro. The device-tip releases stored pheophorebide by attack of singlet oxygen from sensitized oxygen gas delivered through the hollow fiber using 669-nm laser light. The performance of the device was enhanced when configured with a fluorosiliane tip by virtue of its Teflon-like property compared to a conventional glass tip (greater sensitizer quantities photoreleased and laterally diffused, and greater amounts of ovarian OVCAR-5 cancer cell killing). No cell damage was observed at 2.2 N of force applied by the probe tip itself, an amount used for many of the experiments described here.