2H2O-Based HDL Turnover Method for the Assessment of Dynamic HDL Function in Mice

摘要

ObjectiveHigh-density lipoprotein (HDL) promotes reverse cholesterol transport (RCT) from peripheral tissues to the liver for clearance. Reduced HDL-cholesterol (HDLc) is associated with atherosclerosis; however, as a predictor of cardiovascular disease, HDLc has limitations as it is not a direct marker of HDL functionality. Our objective was to develop a mass spectrometry based method for the simultaneous measurement of HDLc and ApoAI kinetics in mice using a single ²H2O tracer, and use it to examine genetic and drug perturbations on HDL turnover in vivo.