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A Noninvasive Approach to Determine Viscoelastic Properties of an Individual Adherent Cell under Fluid Flow

机译:确定流体流动下单个贴壁细胞粘弹性特性的无创方法

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摘要

Mechanical properties of cells play an important role in their interaction with the extracellular matrix as well as the mechanotransduction process. Several in vitro techniques have been developed to determine the mechanical properties of cells, but none of them can measure the viscoelastic properties of an individual adherent cell in fluid flow non-invasively. In this study, techniques of fluid-structure interaction (FSI) finite element method and quasi-3-dimensional (quasi-3D) cell microscopy were innovatively applied to the frequently used flow chamber experiment, where an adherent cell was subjected to fluid flow. A new non-invasive approach, with cells at close to physiological conditions, was established to determine the viscoelastic properties of individual cells. The results showed an instantaneous modulus of osteocytes of 0.49±0.11 kPa, an equilibrium modulus of 0.31±0.044 kPa, and an apparent viscosity coefficient of 4.07±1.23 kPa·s. This new quantitative approach not only provides an excellent means to measure cell mechanical properties, but also may help to elucidate the mechanotransduction mechanisms for a variety of cells under fluid flow stimulation.
机译:细胞的机械性质在它们与细胞外基质的相互作用以及机械转导过程中起着重要作用。已经开发了几种体外技术来确定细胞的机械性能,但是它们中的任何一种都不能非侵入性地测量流体中单个贴壁细胞的粘弹性质。在这项研究中,流固耦合(FSI)有限元法和准3维(准3D)细胞显微镜技术被创新地应用于经常使用的流动室实验,在该实验中,贴壁细胞受到了流体的流动。建立了一种新的非侵入性方法,使细胞处于接近生理条件的状态,以确定单个细胞的粘弹性。结果表明,骨细胞的瞬时模量为0.49±0.11 kPa,平衡模量为0.31±0.044 kPa,表观粘度系数为4.07±1.23 kPa·s。这种新的定量方法不仅为测量细胞的机械性能提供了极好的方法,而且还可能有助于阐明流体流动刺激下各种细胞的机械传导机制。

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