A deep tissue fluorescence imaging system with enhanced SHG detection capabilities

摘要

We describe a novel two-photon fluorescence microscopy system capable of producing high quality SHG images in thick turbid media by using an innovative detection system. This novel detection system is capable of detecting photons from a very large surface area. This system has proven effective in providing images of thick turbid samples, both biological and artificial. Due to its transmission detection geometry, the system is particularly suitable for detecting second harmonic generated signals (SHG) which are generally forward directed. In this paper we present comparative data acquired simultaneously on the same sample with the forward and epi-detection schemes.