Nucleobase-Based Barbiturates: Their Protective Effect against DNA Damage Induced by Bleomycin-Iron Antioxidant and Lymphocyte Transformation Assay

摘要

A number of nucleobase-based barbiturates have been synthesized by combination of nucleic acid bases and heterocyclic amines and barbituric acid derivatives through green and efficient multicomponent route and one pot reaction. This approach was accomplished efficiently using aqueous medium to give the corresponding products in high yield. The newly synthesized compounds were characterized by spectral analysis (FT-IR, ¹H NMR, ¹³C NMR, HMBC, and UV spectroscopy) and elemental analysis. Representative of all synthesized compounds was tested and evaluated for antioxidant, bleomycin-dependent DNA damage, and Lymphocyte Transformation studies. Compounds >TBC > >TBA > >TBG showed highest lymphocyte transformation assay, >TBC > >TBA > >BG showed inhibitory antioxidant activity using ABTS methods, and >TBC > >BPA > >BAMT > >TBA > >1, >3 >-TBA manifested the best protective effect against DNA damage induced by bleomycin.