A point of care test for the determination of amniotic fluid interleukin-6 and the chemokine CXCL-10/IP-10

摘要

ObjectiveIntra-amniotic inflammation is a mechanism of disease implicated in preterm labor, preterm PROM, cervical insufficiency, a short cervix, and idiopathic vaginal bleeding. Determination of interleukin (IL)-6 with immunoassays has been proven for more than 2 decades to be an excellent method for the detection of intra-amniotic inflammation. However, assessment of IL-6 for this indication has been based on immunoassays which are not clinically available, and this has been an obstacle for the implementation of this test in clinical practice. It is now possible to obtain results within 20 minutes with a point of care test which requires minimal laboratory support. This test is based on lateral flow-based immunoassay. The objective of this study was to compare amniotic fluid (AF) IL-6 and interferon-γ –inducible protein 10 (IP-10 or CXCL-10) concentrations determined using lateral flow-based immunoassay or point of care (POC) test and standard enzyme-linked immunosorbent assay (ELISA) techniques.