A Simple and Inexpensive Method to Control Oxygen Concentrations within Physiological and Neoplastic Ranges

摘要

Traditional methods for regulating oxygen concentration ([O2]) in in vitro experiments over the range found in normal and tumor tissues require the use of expensive equipment to generate controlled gas atmospheres, or the purchase of a range of gas cylinders with certified O2 percentages. Herein we describe a simple and inexpensive enzymatic method for generating low, precise steady-state [O2] levels that are stable for several hours. This method is particularly applicable to the in vitro study of some classes of hypoxia targeted antitumor prodrugs and bioreductively activated agents.