Chemical denaturants are the most commonly used perturbation applied to study protein stability and folding kinetics, as well as the properties of unfolded polypeptides. We build on recent work balancing the interactions of proteins and water, and accurate models for the solution properties of urea and guanidinium chloride, to develop a combined force field which is able to capture the strength of interactions between proteins and denaturants. We use solubility data for a model tetraglycine peptide in each denaturant to tune the protein-denaturant interaction by a novel simulation methodology. We validate the results against data for more complex sequences: single molecule Förster resonance energy transfer data for a 34-residue fragment of the globular protein CspTm, and photoinduced electron transfer quenching data for the disordered peptides C(AGQ)nW in denaturant solution, as well as the chemical denaturation of the mini-protein Trp cage. The combined force field model should aid our understanding of denaturation mechanisms and the interpretation of experiment.
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