Multimodal SHG-2PF Imaging of Microdomain Ca2+-Contraction Coupling in Live cardiac myocytes

摘要

Rationalecardiac myocyte contraction is caused by Ca²⁺ binding to troponin C, which triggers the cross-bridge power stroke and myofilament sliding in sarcomeres. Synchronized Ca²⁺ release causes whole cell contraction and is readily observable with current microscopy techniques. However, it is unknown whether localized Ca²⁺ release, such as Ca²⁺ sparks and waves, can cause local sarcomere contraction. Contemporary imaging methods fall short of measuring microdomain Ca²⁺-contraction coupling in live cardiac myocytes.