首页> 美国卫生研究院文献>The Journal of Experimental Medicine >MORPHOLOGICAL AND OTHER CHARACTERISTICS OF THE AGENT OF FELINE PNEUMONITIS GROWN IN THE ALLANTOIC CAVITY OF THE CHICK EMBRYO
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MORPHOLOGICAL AND OTHER CHARACTERISTICS OF THE AGENT OF FELINE PNEUMONITIS GROWN IN THE ALLANTOIC CAVITY OF THE CHICK EMBRYO

机译:鸡胚尿囊空洞中生长的猫肺炎代用品的形态学和其他特征

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摘要

The agent of feline pneumonitis has been grown in the allantoic cavity for 50 serial passages. During this time the amount of agent in allantoic fluid increased about 100-fold. The titer of the agent in the allantoic fluid of the individual embryo reached a peak on the 5th day and then declined. Large inocula were required in order to obtain maximal titers. The toxic factor was present in suspensions of chorioallantoic membranes in allantoic fluid from heavily infected eggs, and could be neutralized by the specific antitoxin produced in rabbits by injection of toxin in yolk sac suspensions. Electron micrographs of the agent of feline pneumonitis grown in the allantoic cavity show that the elementary body is composed of a dense centrally located substance surrounded by a thinner material, part or all of which is the limiting membrane. Separation of these two portions of the body may be due partially or entirely to distortion during drying. The wrinkled surface of the elementary bodies is evidence that such distortion does occur. The average diameter of gold-shadowed bodies is 525 mµ.
机译:猫尿性肺炎病原已在尿囊腔中生长了50次连续传代。在这段时间内,尿囊液中的药剂数量增加了约100倍。单个胚胎尿囊液中药物的效价在第5天达到峰值,然后下降。为了获得最大滴度,需要较大的接种量。毒性因子存在于感染严重的鸡蛋的尿囊液中绒膜尿囊膜的悬浮液中,并可通过在卵黄囊悬浮液中注射毒素而在兔体内产生的特定抗毒素中和。在尿囊腔中生长的猫肺炎病原体的电子显微镜照片显示,基本体由密集的位于中心的物质组成,被较薄的材料包围,部分或全部是限制膜。身体的这两个部分的分离可能部分或完全由于干燥期间的变形而引起。基本体的起皱表面证明确实发生了这种变形。黄金阴影物体的平均直径为525 mµ。

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