Collection of Hematopoietic CD34 Stem Cells in Rhesus Macaques using Spectra Optia

摘要

BackgroundNon-human primates, particularly rhesus macaques, are ideal pre-clinical large animal models to investigate organ tolerance induction protocols using donor hematopoietic stem cells (HSCs) to induce microchimerism. Their relatively small size poses some challenges for the safe and effective collection of peripheral blood HSCs through apheresis procedures. We describe our experiences using the Spectra Optia apheresis unit to successfully obtain HSCs from mobilized peripheral blood of rhesus macaques.