首页> 美国卫生研究院文献>other >The Associative Changes in Scutellum Nuclear Content and Morphology with Viability Loss of Naturally Aged and Accelerated Aging Wheat (Triticum aestivum) Seeds
【2h】

The Associative Changes in Scutellum Nuclear Content and Morphology with Viability Loss of Naturally Aged and Accelerated Aging Wheat (Triticum aestivum) Seeds

机译:自然老化和加速老化小麦种子的盾片核含量和形态与活力丧失的相关变化

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

Timely prediction of seed viability loss over long-term storage represents a challenge in management and conservation of ex situ plant genetic resources. However, little attention has been paid to study the process of seed deterioration and seed aging signals under storage. An attempt was made here to investigate morphological and molecular changes in the scutellum and aleurone sections of naturally or artificially aged wheat seeds using TUNEL assay and DAPI staining. Twelve wheat genotypes or samples exposed to natural ageing (NA) or accelerated ageing (AA) were assayed and these samples had germination rates ranging from 11 to 93%. The assayed samples showed substantial changes in scutellum, but not aleurone. The nuclei observed in the majority of the scutellum cells of the NA seed samples of lower germination rates were longer in size and less visible, while the scutellum cell morphology or arrangement remained unchanged. In contrast, longer AA treatments resulted in the loss of scutellum cell structure, collapse of cell layers, and disappearance of honey comb arrangements. These nuclei and structural changes were consistent with the DNA assessments of nuclear alternations for the selected wheat samples. Interestingly, the sample seed germination loss was found to be associated with the reductions in the scutellum nuclear content and with the increases in the scutellum nuclei length to width ratio. These findings are significant for understanding the process of wheat seed deterioration and are also useful for searching for sensitive seed aging signals for developing tools to monitor seed viability under storage.
机译:及时预测长期保存后种子活力的丧失对异地植物遗传资源的管理和保存提出了挑战。然而,很少有研究存储条件下种子变质和种子老化信号的过程。此处尝试使用TUNEL测定和DAPI染色研究天然或人工老化的小麦种子的盾片和糊粉部分的形态和分子变化。测定了十二种小麦基因型或暴露于自然老化(NA)或加速老化(AA)的样品,这些样品的发芽率范围为11%至93%。被检样品显示盾片有实质性变化,但糊粉没有变化。在较低发芽率的NA种子样品的大多数盾片细胞中观察到的细胞核的大小更长,可见性更差,而盾片细胞的形态或排列保持不变。相反,较长的AA处理导致盾片细胞结构丧失,细胞层塌陷和蜂蜜梳状排列的消失。这些核和结构的变化与所选小麦样品的核交替的DNA评估一致。有趣的是,发现样本种子发芽损失与盾片核含量的减少以及盾片核长宽比的增加有关。这些发现对于理解小麦种子变质的过程具有重要意义,对于寻找敏感的种子老化信号以开发监测贮藏条件下种子生存力的工具也非常有用。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号