Tissue microenvironments in the nasal epithelium of rainbow trout (Oncorhynchus mykiss) define two distinct CD8α+ cell populations and establish regional immunity

摘要

Mucosal surfaces require balancing different physiological roles and immune functions. In order to effectively achieve multifunctionality, mucosal epithelia have evolved unique microenvironments that create unique regional immune responses without impairing other normal physiological functions. Whereas examples of regional immunity are known in other mucosal epithelia, to date, no immune microenvironments have been described in the nasal mucosa, a site where the complex functions of olfaction and immunity need to be orchestrated. In this study we identified for the first time the presence of CD8α⁺ cells in the rainbow trout (Oncorhynchus mykiss) nasal epithelium. Nasal CD8α⁺ cells display a distinct phenotype suggestive of CD8⁺ T cells with high integrin β2 expression. Importantly, nasal CD8α⁺ cells are located in clusters at the mucosal tip of each olfactory lamella but scattered in the neuroepithelial region. The grouping of CD8α⁺ cells may be explained by the greater expression of CCL19, ICAM-1, and VCAM-1 in the mucosal tip compared to the neuroepithelium. Whilst viral antigen uptake occurred via both tip and lateral routes, tip resident MHC-II⁺ cells are located significantly closer to the lumen of the nasal cavity than their neuroepithelial counterparts, therefore having quicker access to invading pathogens. Our studies reveal for the first time compartmentalized mucosal immune responses within the nasal mucosa of a vertebrate species, a strategy that likely optimizes local immune responses while protecting olfactory sensory functions.