GLM-based optimization of NGS data analysis: A case study of Roche 454 Ion Torrent PGM and Illumina NextSeq sequencing data

摘要

BackgroundThere are various next-generation sequencing techniques, all of them striving to replace Sanger sequencing as the gold standard. However, false positive calls of single nucleotide variants and especially indels are a widely known problem of basically all sequencing platforms.