It was proposed that magnetic fields (MFs) can influence gene transcription via CTCT motif located in human HSP70 promoter. To check the universality of this mechanism, we estimated the potential role of this motif on plant gene transcription in response to MFs using both bioinformatics and experimental studies. We searched potential promoter sequences (1000 bp upstream) in the potato Solanum tuberosum and thale cress Arabidopsis thaliana genomes for the CTCT sequence. The motif was found, on average, 3.6 and 4.3 times per promoter (148,487 and 134,361 motifs in total) in these two species, respectively; however, the CTCT sequences were not randomly distributed in the promoter regions but were preferentially located near the transcription initiation site and were closely packed. The closer these CTCT sequences to the transcription initiation site, the smaller distance between them in both plants. One can assume that genes with many CTCT motifs in their promoter regions can be potentially regulated by MFs. To check this assumption, we tested the influence of MFs on gene expression in a transgenic potato with three promoters (16R, 20R, and 5UGT) containing from 3 to 12 CTCT sequences and starting expression of β-glucuronidase as a reported gene. The potatoes were exposed to a 50 Hz 60–70 A/m MF for 30 min and the reporter gene activity was measured for up to 24 h. Although other factors induced the reporter gene activity, the MF did not. It implies the CTCT motif does not mediate in response to MF in the tested plant promoters.
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机译:有人提出磁场(MFs)可以通过位于人HSP70启动子中的CTCT基序影响基因转录。为了检查这种机制的普遍性,我们使用生物信息学和实验研究估计了该基序对植物基因转录响应MF的潜在作用。我们在马铃薯马铃薯和拟南芥拟南芥拟南芥基因组中搜索了潜在的启动子序列(上游1000 bp)以获取CTCT序列。在这两个物种中,每个启动子平均发现该基序分别为3.6和4.3次(总共148,487和134,361个基序);然而,CTCT序列不是随机分布在启动子区域中,而是优先位于转录起始位点附近并且紧密排列。这些CTCT序列越靠近转录起始位点,在两种植物中它们之间的距离越小。可以假定在其启动子区域具有许多CTCT图案的基因可能受到MF的调控。为了检验这个假设,我们测试了MF对具有三个启动子(16R,20R和5UGT)的转基因马铃薯中基因表达的影响,该启动子包含3至12个CTCT序列,并已开始报道β-葡萄糖醛酸苷酶作为已报道的基因。将马铃薯暴露于50 Hz 60-70 A / m MF中30分钟,并测量长达24小时的报告基因活性。尽管其他因素诱导了报告基因的活性,但MF却没有。这暗示了在受试植物启动子中CTCT基序不响应MF而介导。
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