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Cloning optimization of induction conditions and purification of Mycobacterium tuberculosis Rv1733c protein expressed in Escherichia coli

机译：大肠杆菌表达的结核分枝杆菌Rv1733c蛋白的克隆优化诱导条件及纯化

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Background and Objectives:Rv1733c is a latency antigen from Mycobacterium tuberculosis, a probable integral-membrane protein with promiscuous T-cell and B-cell epitopes, making it a potential vaccine candidate against tuberculosis. This study aimed to clone and optimize the expression of recombinant Rv1733c in Escherichia coli for purification.

机译：背景与目的：Rv1733c是一种来自结核分枝杆菌的潜伏期抗原，它是一种可能的整合膜蛋白，具有混杂的T细胞和B细胞表位，使其成为抗结核疫苗的潜在候选者。这项研究旨在克隆和优化重组Rv1733c在大肠杆菌中的表达以进行纯化。

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期刊名称 other
作者
Mitra Ashayeri-Panah; Fereshteh Eftekhar; Bahram Kazemi; Joan Joseph;
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年(卷),期 -1(9),2
年度 -1
页码 64–73
总页数 10
原文格式 PDF
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关键词
Mycobacterium tuberculosis Rv1733c Cloning Expression optimization Protein purification;

机译：结核分枝杆菌;Rv1733c;克隆;表达优化;蛋白质纯化;

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专利

1. Cloning, optimization of induction conditions and purification of Mycobacterium tuberculosis Rv1733c protein expressed in Escherichia coli [J] . Mitra Ashayeri-Panah, Fereshteh Eftekhar, Bahram Kazemi, Iranian Journal of Microbiology . 2017,第2期

机译：大肠杆菌表达的结核分枝杆菌Rv1733c蛋白的克隆，优化诱导条件及纯化
2. Amplification of six putative RD1 genes of Mycobacterium tuberculosis for cloning and expression in Escherichia coli and purification of expressed proteins. [J] . Amoudy HA, Mustafa AS Medical principles and practice: international journal of the Kuwait University, Health Science Centre . 2008,第5期

机译：结核分枝杆菌的六个推定的RD1基因的扩增，用于在大肠杆菌中的克隆和表达以及表达蛋白的纯化。
3. Construction of a modified vector for efficient purification of recombinant Mycobacterium tuberculosis proteins expressed in Escherichia coli [J] . Ahmad S., Ali MM., Mustafa AS. Protein Expression and Purification . 2003,第2期

机译：用于有效纯化在大肠杆菌中表达的重组结核分枝杆菌蛋白的修饰载体的构建
4. Cloning and Expression of 34KDa Protein Gene of Mycobacterium paratuberculosis in Escherichia coli [C] . Jiang Xiu-Yun, Wang Chun-Fang, Zeng Fan-Li, 2010 4th International Conference on Bioinformatics and Biomedical Engineering . 2010

机译：副结核分枝杆菌34KDa蛋白基因的克隆与表达
5. Heterodimeric deoxyguanosine/deoxyadenosine kinase from Lactobacillus acidophilus R-26: Affinity protein purification, molecular cloning, sequence of the genes, and expression in Escherichia coli. [D] . Ma, Grace Tak-Yi. 1993

机译：嗜酸乳杆菌R-26的异二聚体脱氧鸟苷/脱氧腺苷激酶：亲和蛋白纯化，分子克隆，基因序列和在大肠杆菌中的表达。
6. Mycobacterium tuberculosis HspX/EsxS Fusion Protein: Gene Cloning Protein Expression and Purification in Escherichia coli [O] . Farzad Khademi, Arshid Yousefi-Avarvand, Mohammad Derakhshan, 2017

机译：结核分枝杆菌HspX / EsxS融合蛋白：大肠杆菌中的基因克隆蛋白表达和纯化
7. Cloning and expression of multiple integral membrane proteins from Mycobacterium tuberculosis in Escherichia coli [O] . Korepanova, Alla, Gao, Fei P., Hua, Yuanzi, 2009

机译：结核分枝杆菌中多种整合膜蛋白的克隆与表达

Cloning optimization of induction conditions and purification of Mycobacterium tuberculosis Rv1733c protein expressed in Escherichia coli

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