The mitochondrial Na+/Ca2+exchanger is essential for Ca2+ homeostasis andviability

摘要

Mitochondrial calcium (mCa²⁺) has a central role in both metabolic regulation and cell death signalling, however its role in homeostatic function and disease is controversial. Slc8b1 encodes the mitochondrial Na⁺/Ca²⁺ exchanger (NCLX), which is proposed to be the primary mechanism for mCa²⁺ extrusion in excitable cells^,. Here we show that tamoxifen-induced deletion of Slc8b1 in adult mouse hearts causes sudden death, with less than 13% of affected mice surviving after 14 days. Lethality correlated with severe myocardial dysfunction and fulminant heart failure. Mechanistically, cardiac pathology was attributed to mCa²⁺ overload driving increased generation of superoxide and necrotic cell death, which was rescued by genetic inhibition of mitochondrial permeability transition pore activation. Corroborating these findings, overexpression of NCLX in the mouse heart by conditional transgenesis had the beneficial effect of augmenting mCa²⁺ clearance, preventing permeability transition and protecting against ischaemia-induced cardiomyocyte necrosis and heart failure. These results demonstrate the essential nature ofmCa²⁺ efflux in cellular function and suggestthat augmenting mCa²⁺ efflux may be a viabletherapeutic strategy in disease.