Cell density DMSO concentration and needle gauge affect hydrogel-induced BM-MSC viability

摘要

Mesenchymal stromal cells (MSCs) have shown potential therapeutic benefits for a range of medical disorders and maintain a focus of intense scientific investigation. Transplantation of MSCs into injured tissue can improve wound healing, tissue regeneration and functional recovery. However, implanted cells rapidly lose their viability or fail to integrate into host tissue. Hydrogel-seeded BM-MSCs offer improved viability in response to mechanical forces caused by syringe needles, cell density and dimethylsulfoxide (DMSO) concentration, which in turn, will help to clarify which factors are important for enhancing biomaterial-induced cell transplantation efficiency and provide much needed guidance for clinical trials. In this study, under the control of cell density (lower than 2×10⁷ cells/ml) and final DMSO concentration (lower than 0.5%), hydrogel-induced BM-MSC viability remained over 82% following syringe needle passage by 25 or 27 gauge needles, providing improved cell therapeutic approaches for regenerative medicine.