Time-Gated Luminescence Detection of Enzymatically Produced Hydrogen Sulfide: Design Synthesis and Application of a Lanthanide-Based Probe

摘要

Hydrogen sulfide (H2S) is now recognized as an important gaseous transmitter that is involved in a variety of biological processes. Here, we report the design and synthesis of a luminescent lanthanide biosensor for H2S, LP2-Cu(II)-Ln(III), a heterobinuclear metal complex that uses Cu(II) decomplexation to control ms-scale lifetime Tb(III) or Eu(III) emission intensity. LP2-Cu(II)-Ln(III) responded rapidly, selectively and with high sensitivity to aqueous H2S. The probe’s potential for biological applications was verified by measuring the H2S generated by the slow-releasing chemical sulfide donor GYY4147, by cystathionine γ-lyase (CSE) and by Na2S-stimulated HeLa cells.