The large intestine (cecum and colon) is a complex biochemical factory of vital importance to human health. It plays a major role in digestion and absorption by salvaging nutrients from polysaccharides via fermentation initiated by the bacteria that comprise the gut microbiome. We hypothesize that the intestinal epithelium absorbs a limited number of luminal metabolites with bioactive potential while actively excluding those with toxic effects. To explore this concept, we combined 1H NMR detection with Ussing chamber measurements of absorptive transport by rat cecum. Numerous metabolites transported across the epithelium can be measured simultaneously by 1H NMR, a universal detector of organic compounds, alleviating the need for fluorescent or radiolabeled compounds. Our results demonstrate the utility of this approach to delineate the repertoire of fecal solutes that are selectively absorbed by the cecum and to determine their transport rates.
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机译：大肠（盲肠和结肠）是一个复杂的生化工厂，对人类健康至关重要。它通过组成肠道微生物组的细菌引发的发酵从多糖中挽救营养，从而在消化和吸收中起主要作用。我们假设肠道上皮吸收具有生物活性潜力的有限数量的腔内代谢产物，同时积极地排除具有毒性作用的腔内代谢产物。为了探索这一概念，我们将 1 1H NMR检测与用于盲肠吸收性转运的Ussing室测量相结合。通过 1 1 H NMR（一种有机化合物的通用检测器），可以同时测量跨上皮运输的许多代谢产物，从而减少了对荧光或放射性标记化合物的需求。我们的结果表明，该方法可用于描绘盲肠选择性吸收的粪便溶质库，并确定其转运速率。
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