Automated radiosynthesis of 18FFBEM a sulfhydryl sitespecific labeling agent for peptides and proteins

摘要

In the process of developing [¹⁸F]FBEM coupled target peptide, we have instituted a robust automated synthesis of [¹⁸F]FBEM, a sulfhydryl (-SH) site specific agent for radiolabeling of peptides and proteins. The radiosynthesis generated 1.67–3.89 GBq (45.1–105.1 mCi, 7.5–18.8% non-decay corrected yield) of [¹⁸F]FBEM from 22.2 GBq (600 mCi) of starting [¹⁸F]fluoride with molar activity of 31.8±5.3 GBq/µmol (0.86±0.14 mCimol) (n=3) at the end of synthesis. Radiochemical purity was greater than 98%, and total synthesis time was ~90 min.