In Vitro and in Vivo Studies of Chlorin-based Carbon Nanocarrier with Photodynamic Therapy Features

摘要

Carbonaceous materials have long been developed to utilize “nano-spaces” and numerous guest species could be encapsulated. A remarkable fluorescence difference has been observed after newly-designed pyropheophorbide-a-appended carbon nanohorns were incorporated into cellular medium and confocal microscopy was employed for the determination of the intracellular localization. Our study supported the role for carbon nanohorns as carriers of photodynamic therapy (PDT) agent and its heating behavior were discussed. We have developed the theranostic platform based on photosensitizer-conjugated carbon nanostructure and this system has been applied in an animal model. In addition, a negligible toxicity of CNH-Pyro was found in body weight experiments and histopathological examination of major organs.