A fungal strain, Penicillium purpurogenum IAM 15392, produced the azaphilone Monascus pigment homolog when cultured in a medium composed of soluble starch, ammonium nitrate, yeast extract, and citrate buffer, pH 5.0. One of the typical features of violet pigment PP-V [(10Z)-12- carboxyl-monascorubramine] is that pyranoid oxygen is replaced with nitrogen. In this study, we found that glutamine synthetase (glnB) and glutamate dehydrogenase (gdh1) genes were expressed in the culture conditions conducive to PP-V production. Gln and Glu both support PP-V biosynthesis, but PP-V biosynthesis was much more efficient with Gln. We determined that synthesis of Gln by glutamine synthetase from ammonium is important for PP-V production.
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机译:当在由可溶性淀粉,硝酸铵,酵母提取物和柠檬酸盐缓冲液组成的培养基中(pH≥5.0)进行培养时,一种真菌菌株Puricillium purpurogenum IAM 15392产生了azaphilone红曲霉色素同源物。紫罗兰色颜料PP-V [(10Z)-12-羧基-单萘酷胺]的典型特征之一是吡喃类化合物的氧被氮取代。在这项研究中,我们发现谷氨酰胺合成酶(glnB)和谷氨酸脱氢酶(gdh1)基因在有利于PP-V生产的培养条件下表达。 Gln和Glu都支持PP-V的生物合成,但是PP-V的生物合成对Gln更为有效。我们确定,由铵中的谷氨酰胺合成酶合成Gln对PP-V的生产很重要。
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