In this work a defined co-culture of the lactic acid bacterium Lactobacillus zeae and the propionate producer Veillonella criceti has been studied in continuous stirred tank reactor (CSTR) and in a dialysis membrane reactor. It is the first time that this reactor type is used for a defined co-culture fermentation. This reactor allows high mixing rates and working with high cell densities, making it ideal for co-culture investigations. In CSTR experiments the co-culture showed over a broad concentration range an almost linear correlation in consumption and production rates to the supply with complex nutrients. In CSTR and dialysis cultures a strong growth stimulation of L. zeae by V. criceti was shown. In dialysis cultures very high propionate production rates (0.61 g L-1h-1) with final titers up to 28 g L-1 have been realized. This reactor allows an individual, intracellular investigation of the co-culture partners by omic-technologies to provide a better understanding of microbial communities.
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机译:在这项工作中,已经在连续搅拌釜反应器(CSTR)和透析膜反应器中研究了乳酸细菌zeabacillus zeae和丙酸生产者Veillonella criceti的明确共培养。这是该反应器类型首次用于确定的共培养发酵。该反应器可实现高混合速率并能在高细胞密度下工作,使其成为共培养研究的理想选择。在CSTR实验中,共培养表明,在很宽的浓度范围内,消耗量和生产率与复杂营养素的供给几乎呈线性关系。在CSTR和透析培养物中,显示了强烈的生长刺激。在透析培养物中,丙酸的生产速率非常高(0.61 g L -1 sup> h -1 sup>),最终滴度可达28 g L -1 sup>被实现。该反应器允许通过眼科学技术对共培养伙伴进行单独的细胞内研究,以更好地了解微生物群落。
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