OLE (Ornate, Large, Extremophilic) RNAs represent a recently discovered non-coding RNA class found in extremophilic anaerobic bacteria, including certain human pathogens. OLE RNAs exhibit several unusual characteristics that indicate a potentially novel function, including exceptionally high expression and localization to cell membranes via interaction with a protein partner called OLE-associated protein (OAP). In the current study, new genetic and phenotypic characteristics of OLE RNA from Bacillus halodurans C-125 were established. OLE RNA is transcribed at high levels from its own promoter under normal growth conditions and the transcript is exceptionally stable compared to most other RNAs. Expression is increased by ∼7-fold when cells are exposed to near lethal concentrations of short-chain alcohols such as ethanol or methanol. Strains wherein the genes for OLE and/or OAP are deleted are more susceptible to growth inhibition by alcohol and also become more sensitive to cold. Normal growth characteristics can be restored by expressing the genes for OLE and OAP from plasmids or from elsewhere on the chromosome. Our findings confirm a functional link between OLE and OAP and reveal the importance of a large non-coding RNA in the response to alcohol-induced stress.
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机译:OLE(华丽,大型,极端嗜热)RNA代表最近发现的极端嗜性厌氧细菌(包括某些人类病原体)中的非编码RNA类。 OLE RNA具有几个不寻常的特征,这些特征指示潜在的新功能,包括异常高的表达和通过与称为OLE相关蛋白(OAP)的蛋白伴侣的相互作用而定位于细胞膜。在当前的研究中,建立了来自嗜盐芽孢杆菌C-125的OLE RNA的新的遗传和表型特征。 OLE RNA在正常生长条件下从其自身的启动子高水平转录,与大多数其他RNA相比,转录本异常稳定。当细胞暴露于接近致命浓度的短链醇(例如乙醇或甲醇)中时,表达增加约7倍。 OLE和/或OAP基因缺失的菌株更容易受到酒精的生长抑制,并且对寒冷也变得更加敏感。可以通过从质粒或染色体上的其他地方表达OLE和OAP的基因来恢复正常的生长特性。我们的发现证实了OLE和OAP之间的功能联系,并揭示了大的非编码RNA在酒精诱导的应激反应中的重要性。
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