Functional expression of calcium‐permeable canonical transient receptor potential 4‐containing channels promotes migration of medulloblastoma cells

摘要

Key points class="unordered" style="list-style-type:disc" id="tjp12483-list-0001">The proton sensing ovarian cancer G protein coupled receptor 1 (OGR1, aka GPR68) promotes expression of the canonical transient receptor potential channel subunit TRPC4 in normal and transformed cerebellar granule precursor (DAOY) cells.OGR1 and TRPC4 are prominently expressed in healthy cerebellar tissue throughout postnatal development and in primary cerebellar medulloblastoma tissues.Activation of TRPC4‐containing channels in DAOY cells, but not non‐transformed granule precursor cells, results in prominent increases in [Ca²⁺]i and promotes cell motility in wound healing and transwell migration assays.Medulloblastoma cells not arising from granule precursor cells show neither prominent rises in [Ca²⁺]i nor enhanced motility in response to TRPC4 activation unless they overexpressTRPC4.Our results suggest that OGR1 enhances expression of TRPC4‐containing channels that contribute to enhanced invasion and metastasis of granule precursor‐derived human medulloblastoma.