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Rapid depth-resolved light scattering measurements using Fourier domain angle-resolved low coherence interferometry

机译:使用傅立叶域角度分辨低相干干涉法进行快速深度分辨的光散射测量

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摘要

We present a novel angle-resolved low coherence interferometry scheme for rapid measurement of depth-resolved angular scattering distributions to enable determination of scatterer size via elastic scattering properties. Depth resolution is achieved using a superluminescent diode in a modified Mach-Zehnder interferometer with the mixed signal and reference fields dispersed by an imaging spectrograph. The spectrograph slit is located in a Fourier transform plane of the scattering sample, enabling angle-resolved measurements over a 0.21 radian range. The capabilities of the new technique are demonstrated by recording the distribution of light scattered by a sub-surface layer of polystyrene microspheres in 40 milliseconds. The data are used to determine the microsphere size with good accuracy. Future clinical application to measuring the size of cell nuclei in living epithelial tissues using backscattered light is discussed.
机译:我们提出了一种新颖的角度分辨低相干干涉测量方案,用于快速测量深度分辨角散射分布,从而能够通过弹性散射特性确定散射体的大小。使用改进的Mach-Zehnder干涉仪中的超发光二极管可实现深度分辨率,混合信号和参考场由成像光谱仪分散。光谱仪狭缝位于散射样品的傅立叶变换平面中,可在0.21弧度范围内进行角度分辨测量。通过记录40毫秒内聚苯乙烯微球表面下层散射的光的分布,证明了这项新技术的功能。数据用于确定微球尺寸,且精度较高。讨论了使用反向散射光测量活体上皮组织中细胞核大小的未来临床应用。

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