We demonstrate a hyperspectral and depth sensitive diffuse optical imaging microsystem, where fast scanning is provided by a CMOS compatible 2-axis MEMS mirror. By using lissajous scanning patterns, large field-of-view (FOV) of 1.2cm x 1.2cm images with lateral resolution of 100µm can be taken at 1.3 frames-per-second (fps). Hyperspectral and depth-sensitive images were acquired on tissue simulating phantom samples containing quantum dots (QDs) patterned at various depths in Polydimethylsiloxane (PDMS). Device performance delivers 6 nm spectral resolution and 0.43 wavelengths per second acquisition speed. A sample of porcine epithelium with subcutaneously placed QDs was also imaged. Images of the biological sample were processed by spectral unmixing in order to qualitatively separate chromophores in the final images and demonstrate spectral performance of the imaging system.
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机译：我们演示了一个高光谱和深度敏感的漫射光学成像微系统，其中通过兼容CMOS的2轴MEMS反射镜提供快速扫描。通过使用lissajous扫描模式，可以以1.3帧/秒（fps）的速度拍摄1.2cm x 1.2cm图像的大视野（FOV），横向分辨率为100μm。在模拟包含在聚二甲基硅氧烷（PDMS）的不同深度图案化的量子点（QD）的体模样品的组织上获取高光谱和深度敏感图像。器件性能可提供6 nm的光谱分辨率和每秒0.43个波长的采集速度。还对具有皮下放置的QD的猪上皮样品进行了成像。通过光谱解混处理生物样品的图像，以定性分离最终图像中的生色团，并证明成像系统的光谱性能。
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