We detected single living bacterial cells on ultramicroelectrode (UME) using a single-particle collision method and optical microscopic methods. The number of collision events involving the bacterial cells indicated in current-time (i-t) curves corresponds to the number of bacterial cells (i.e., Escherichia coli) on the UME surface, as observed visually. Simulations were performed to determine the theoretical current response (75 pA) and frequency (0.47 pM−1 s−1) of single Escherichia coli collisions. The experimental current response (83 pA) and frequency (0.26 pM−1 s−1) were on the same order of magnitude as the theoretical values. This single-particle collision approach facilitates detecting living bacteria and determining their concentration in solution and could be widely applied to studying other bacteria and biomolecules.
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机译：我们使用单粒子碰撞方法和光学显微镜方法在超微电极（UME）上检测到了单个活细菌细胞。电流-时间（i-t）曲线中指示的涉及细菌细胞的碰撞事件的数量对应于UME表面上的细菌细胞（即，大肠杆菌）的数量，如在视觉上观察到的。进行模拟以确定单个大肠杆菌碰撞的理论电流响应（75 pA）和频率（0.47 pM -1 s -1 ）。实验电流响应（83 pA）和频率（0.26 pM -1 s -1 ）与理论值处于同一数量级。这种单粒子碰撞方法有助于检测活细菌并确定其在溶液中的浓度，可广泛用于研究其他细菌和生物分子。
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