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>Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
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Interaction studies of carbon nanomaterials and plasma activated carbon nanomaterials solution with telomere binding protein
Most cancer cells have telomerase activity because they can express the human telomerase reverse transcriptase (hTERT) gene. Therefore, the inhibition of the hTERT expression can play an important role in controlling cancer cell proliferation. Our current study aims to inhibit hTERT expression. For this, we synthesized graphene oxide (GO) and a functionalized multiwall carbon nanotube (f-MWCNT), latter treated them with cold atmospheric pressure plasma for further analysis of the hTERT expression. The inhibition of hTERT expression by GO, f-MWCNT, plasma activated GO solution (PGOS), and plasma activated f-MWCNT solution (PCNTS), was studied using two lung cancer cell lines, A549 and H460. The hTERT experimental results revealed that GO and PGOS sufficiently decreased the hTERT concentration, while f-MWCNT and PCNTS were unable to inhibit the hTERT concentration. Therefore, to understand the inhibition mechanism of hTERT, we studied the binding properties of GO and PGOS with telomere binding protein (AtTRB2). The interaction studies were carried out using circular dichroism, fluorescence, 1H-15N NMR spectroscopy, and size-exclusion chromatography (SEC) binding assay. We also used docking simulation to have an better understanding of the interactions between GO nanosheets and AtTRB2 protein. Our results may provide new insights that can benefit in biomedical treatments.
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机译:大多数癌细胞具有端粒酶活性,因为它们可以表达人类端粒酶逆转录酶(hTERT)基因。因此,抑制hTERT表达可以在控制癌细胞增殖中起重要作用。我们当前的研究旨在抑制hTERT表达。为此,我们合成了氧化石墨烯(GO)和功能化的多壁碳纳米管(f-MWCNT),然后用冷大气压等离子体对其进行处理,以进一步分析hTERT表达。使用两种肺癌细胞系A549和H460研究了GO,f-MWCNT,血浆活化的GO溶液(PGOS)和血浆活化的f-MWCNT溶液(PCNTS)对hTERT表达的抑制作用。 hTERT实验结果表明,GO和PGOS足以降低hTERT浓度,而f-MWCNT和PCNTS无法抑制hTERT浓度。因此,为了了解hTERT的抑制机制,我们研究了GO和PGOS与端粒结合蛋白(AtTRB2)的结合特性。使用圆二色性,荧光, 1 sup> H- 15 sup> N NMR光谱和尺寸排阻色谱(SEC)结合分析进行了相互作用研究。我们还使用了对接模拟,以更好地了解GO纳米片与AtTRB2蛋白之间的相互作用。我们的结果可能会提供新的见识,从而可以在生物医学治疗中受益。
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