Expression of RUNX3 gene and miR-363 in colorectal cancer was studied to explore its relationship with clinicopathological characteristics of colorectal cancer and to analyze the value of RUNX3 combined with miR-363 in the diagnosis of colorectal cancer. In total, 85 patients diagnosed with colorectal cancer in the First Peoples Hospital of Xiaoshan Hangzhou from March 2014 to July 2016 were the experiment group. Seventy healthy individuals who underwent physical examination were the control group. RT-qPCR was used to detect the expression levels of RUNX3 gene and miR-363 in peripheral blood of the two groups. The relationship between the expression of RUNX3 and miR-363 with its clinicopathological characteristics was analyzed as well. The expression of RUNX3 in the experiment group was significantly lower than that in the control group (P<0.05). The expression level of miR-363 was significantly lower than in the control group (P<0.05). However, there was a correlation with tumor size, degree of differentiation, lymph node metastasis, depth of invasion and clinical stages (P<0.05). RUNX3 and miR-363 were significantly positively correlated with the degree of differentiation (r=0.7381, r=0.5375; P<0.05); RUNX3 and miR-363 were significantly negatively correlated with clinical stages (r=−0.7167, −0.6700; P<0.05). The area under the ROC curve of the combined test was larger than the single test. The expression of RUNX3 gene and miR-363 in peripheral blood of patients with colorectal cancer was lower than in the normal controls. The low expression of RUNX3 and miR-363 was closely related to various biological behaviors of colorectal cancer. A potential reference is provided for the evaluation of patients with colorectal cancer and expected to have an important guiding effect in the treatment of colorectal cancer. Moreover, combined test of RUNX3 and miR-363 has important significance in the diagnosis and treatment evaluation of colorectal cancer.
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机译：研究RUNX3基因和miR-363在大肠癌中的表达，探讨其与大肠癌临床病理特征的关系，探讨RUNX3联合miR-363在大肠癌诊断中的价值。实验组为2014年3月至2016年7月在杭州市萧山市第一人民医院诊断为大肠癌的85例患者。进行健康检查的健康个体为70名。 RT-qPCR检测两组外周血RUNX3基因和miR-363的表达水平。还分析了RUNX3和miR-363的表达与其临床病理特征的关系。实验组RUNX3的表达明显低于对照组（P <0.05）。 miR-363的表达水平明显低于对照组（P <0.05）。但与肿瘤大小，分化程度，淋巴结转移，浸润深度和临床分期有相关性（P <0.05）。 RUNX3和miR-363与分化程度呈显着正相关（r = 0.7381，r = 0.5375； P <0.05）。 RUNX3和miR-363与临床分期显着负相关（r = -0.7167，-0.6700; P <0.05）。组合测试的ROC曲线下面积大于单个测试。大肠癌患者外周血中RUNX3基因和miR-363的表达低于正常对照组。 RUNX3和miR-363的低表达与大肠癌的多种生物学行为密切相关。为评估结直肠癌患者提供了潜在的参考，有望在治疗结直肠癌方面具有重要的指导作用。而且，RUNX3和miR-363的联合检测对大肠癌的诊断和治疗评价具有重要意义。
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