EFFECTS OF ADMINISTRATION ROUTE DIETARY CONDITION AND BLOOD GLUCOSE LEVEL ON KINETICS AND UPTAKE OF 18F-FDG IN MICE

摘要

The effects of dietary condition and blood glucose level on the kinetics and uptake of ¹⁸F-FDG in mice were systematically investigated using intraperitoneal and tail-vein injection.MethodsDynamic PET was performed for 60 min on 23 isoflurane-anesthetized male C57BL/6 mice after intravenous (n = 11) or intraperitoneal (n = 12) injection of ¹⁸F-FDG. Five and 6 mice in the intravenous and intraperitoneal groups, respectively, were kept fasting overnight (18 ± 2 h), and the others were fed ad libitum. Serial blood samples were collected from the femoral artery to measure ¹⁸F-FDG and glucose concentrations. Image data were reconstructed using filtered backprojection with CT-based attenuation correction. The standardized uptake value (SUV) was estimated from the 45- to 60-min image. The metabolic rate of glucose (MRGlu) and ¹⁸F-FDG uptake constant (Ki) were derived by Patlak graphical analysis.