首页> 美国卫生研究院文献>Journal of Medical Entomology >Relationships Between Infection Dissemination and Transmission of West Nile Virus RNA in Culex pipiens quinquefasciatus (Diptera: Culicidae)
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Relationships Between Infection Dissemination and Transmission of West Nile Virus RNA in Culex pipiens quinquefasciatus (Diptera: Culicidae)

机译:西尼罗罗非鱼库蚊(Diptera:Culicidae)中的西尼罗河病毒RNA的感染传播和传播之间的关系。

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摘要

Culex pipiens quinquefasciatus Say fed blood containing 6.8 ± 0.3 logs (mean ± SE) plaque-forming units of West Nile virus (WNV)/ml were maintained at 28°C for incubation periods (IP) of 7, 14, or 21 d. Several attributes of vector competence were determined at each IP using quantitative real-time reverse transcriptase polymerase chain reaction to estimate plaque forming unit equivalents including: infection rate (WNV-positive abdomens), dissemination rate (WNV-positive legs or thoraces), combined dissemination rate (WNV-positive legs and thoraces), transmission rate (WNV-positive saliva), and WNV titers in abdomens, legs, thoraces, and saliva. Each rate increased or was equivalent with increasing IP. Mosquitoes transmitting WNV in saliva also had significantly higher IP-dependent WNV titers in abdomens, legs, and thoraces. Titers of WNV in abdomens were significantly correlated with titers in legs and thoraces, but the degree of association changed with IP. However, titers of abdomens, legs, and thoraces were not correlated with WNV presence or titer in the saliva. The results show that WNV presence or titer in the saliva of infected Cx. p. quinquefasciatus was not directly influenced by processes involved in WNV replication in other tissues. The processes controlling midgut infection and escape are, in part, independent from the infection processes in other tissues. The relationship between infection, dissemination, and transmission varied over time. The infection and replication of WNV in different tissues is likely influenced by different barriers encountered during the extrinsic incubation period. The significance of these observations for understanding vector competence is discussed.
机译:饲喂含6.8±0.3 log(mean±SE)斑块形成单位的西尼罗河病毒(WNV)/ ml的进食血液在28°C下保持7 d,14 d或21 d的潜伏期。使用定量实时逆转录酶聚合酶链反应在每个IP上确定载体能力的几个属性,以估计噬菌斑形成单位当量,包括:感染率(WNV阳性腹部),传播率(WNV阳性腿或胸廓),联合传播腹部,腿部,胸部和唾液中的WNV阳性率(WNV阳性腿和胸廓),WNV阳性传播率(WNV阳性唾液)。每个速率随IP的增加而增加或相等。在唾液中传播WNV的蚊子在腹部,腿部和胸部也具有明显更高的IP依赖WNV滴度。腹部WNV滴度与腿部和胸部的滴度显着相关,但关联程度随IP而变化。然而,腹部,腿部和胸腔的滴度与唾液中存在NVV或滴度无关。结果表明感染的Cx唾液中存在WNV或滴度。 p。 quinquefasciatus不受其他组织中WNV复制过程的直接影响。控制中肠感染和逃逸的过程部分独立于其他组织的感染过程。感染,传播和传播之间的关系随时间而变化。 WNV在不同组织中的感染和复制很可能受外在潜伏期遇到的不同障碍的影响。讨论了这些观察对于理解向量能力的意义。

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