Streptomycesheilongjiangensis sp. nov. a novel actinomycete that produces borrelidin isolated from the root surface of soybean Glycine max (L.) Merr

摘要

A borrelidin-producing actinomycete, designated strain NEAU-W2^T, was isolated from the root surface of soybean [Glycine max (L.) Merr] and characterized using a polyphasic approach. The organism was found to have morphological and chemotaxonomic characteristics typical of streptomycetes. The G+C content of the DNA was 66.12 mol%. Analysis of the 16S rRNA gene sequence of strain NEAU-W2^T revealed that the strain formed a distinct clade within the 16S rRNA gene sequence phylogenetic tree and showed highest similarity (99.61 %) to ATCC 27449^T. However, the DNA–DNA relatedness between strain NEAU-W2^T and ATCC 27449^T was 58.51 %. Strain NEAU-W2^T could also be differentiated from ATCC 27449^T and other species showing high 16S rRNA gene sequence similarity (98–99 %), as well as other borrelidin-producing strains, based on morphological and physiological characteristics. On the basis of its physiological and molecular properties, it is proposed that strain NEAU-W2^T represents a novel species, heilongjiangensis sp. nov. The type strain is NEAU-W2^T ( = CGMCC 4.7004^T  = ATCC BAA-2424^T  = DSM 42073^T).