Stenotrophomonas bentonitica sp. nov. isolated from bentonite formations

摘要

A Gram-stain negative, rod-shaped, aerobic bacterial strain, BII-R7^T, was isolated during a study targeting the culture-dependent microbial diversity occurring in bentonite formations from southern Spain. Comparative 16S rRNA gene sequence analysis showed that BII-R7^T represented a member of the genus (class ), and was related most closely to e-p10^T (99.2 % sequence similarity), followed by ICB 89^T (98.5 %), IAM 12423^T, LPM-5^T and T5916-2-1b^T (all 98.3 %). Pairwise sequence similarities to all other type strains of species of the genus were below 98 %. Genome-based calculations (orthologous average nucleotide identity, original average nucleotide identity, genome-to-genome distance and DNA G+C percentage) indicated clearly that the isolate represents a novel species within this genus. Different phenotypic analyses, such as the detection of a quinone system composed of the major compound ubiquinone Q-8 and a fatty acid profile with iso-C15 : 0 and anteiso-C15 : 0 as major components, supported this finding at the same time as contributing to a comprehensive characterization of BII-R7^T. Based on this polyphasic approach comprising phenotypic and genotypic/molecular characterization, BII-R7^T can be differentiated clearly from its phylogenetic neighbours, establishing a novel species for which the name Stenotrophomonas bentonitica sp. nov. is proposed with BII-R7^T as the type strain (=LMG 29893^T=CECT 9180^T=DSM 103927^T).