The aim of this study was to evaluate the effects of a proanthocyanidin-rich grape seed extract (GSE) on the in vitro demineralization of root dentine. Root fragments were obtained from sound human teeth. The fragments were randomly assigned to different treatments solutions: GSE, fluoride (F), GSE+F and distilled water (control). Samples were treated daily for 30 min and subjected to a pH cycling artificial caries protocol using demineralization cycles (2.2 mM CaCl2×H2O, 2.2 mM KH2PO4, 50 mM acetic acid, pH 4.3) for 6 h and remineralization cycles (20 mM HEPES, 2.25 mM CaCl2×H2O, 1.35 mM KH2PO4, 130 mM KCl, pH 7.0) for 17.5 h. Mineral loss (ΔZ) and lesion depth (LD) were determined after 18 days of treatment/pH cycling, by transverse microradiography. GSE was able to minimize ΔZ and LD compared with the control group (p < 0.0001). The GSE+F and F groups showed the lowest values of ΔZ and LD (p < 0.05), with no statistically significant differences between them (p = 0.554 and p = 0.726, respectively). A biomimetic approach to strengthen root dentine using GSE results in decreased rates of root demineralization and may be used in conjunction with F to prevent root caries.
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机译：这项研究的目的是评估富含原花青素的葡萄籽提取物（GSE）对牙本质牙本质体外脱矿质的影响。根碎片是从健全的人类牙齿获得的。将片段随机分配给不同的处理溶液：GSE，氟化物（F），GSE + F和蒸馏水（对照）。每天将样品处理30分钟，并使用去矿质循环（2.2 mM CaCl2×H2O，2.2 mM KH2PO4、50 mM乙酸，pH 4.3）进行pH循环人工龋齿实验6小时和再矿化循环（20 mM HEPES，2.25） mM CaCl 2×H 2 O，1.35 mM KH 2 PO 4，130 mM KCl，pH 7.0）持续17.5小时。在治疗/ pH循环18天后，通过横向显微X射线照相术测定矿物质损失（ΔZ）和病变深度（LD）。与对照组相比，GSE能够最小化ΔZ和LD（p <0.0001）。 GSE + F和F组的ΔZ和LD值最低（p <0.05），它们之间没有统计学上的显着差异（分别为p = 0.554和p = 0.726）。使用GSE强化根部牙本质的仿生方法可降低根部脱矿质的速度，可与F结合使用以预防根部龋齿。
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