We present particle counting ultrahigh-resolution optical Doppler tomography (pc-μODT) that enables accurate imaging of red blood cell velocities (νRBC) of cerebrovascular networks by detecting the Doppler phase transients induced by the passage of a RBC through a capillary. We apply pc-μODT to image the response of capillary νRBC to mild hypercapnia in mouse cortex. The results show that νRBC in normocapnia (νN = 0.72 ± 0.15 mm/s) increased 36.1% ± 5.3% (νH = 0.98 ± 0.29 mm/s) in response to hypercapnia. Due to uncorrected angle effect and low hematocrit (e.g., ∼10%), νRBC directly measured by μODT were markedly underestimated (νN ≈ 0.27 ± 0.03 mm/s, νH ≈ 0.37± 0.05 mm/s). Nevertheless, the measured νRBC increase (35.3%) matched that (36.1% ± 5.3%) by pc-μODT.
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机译：我们提出了粒子计数超高分辨率光学多普勒层析成像（pc-μODT），通过检测RBC穿过毛细血管而引起的多普勒相位瞬变，从而能够对脑血管网络的红细胞速度（νRBC）进行精确成像。我们应用pc-μODT成像毛细管vRBC对小鼠皮质轻度高碳酸血症的反应。结果表明，正常人高碳酸血症中的vRBC（vN = 0.72±0.15 mm / s）增加36.1％±5.3％（νH= 0.98±0.29 mm / s）。由于未校正的角度效应和较低的血细胞比容（例如〜10％），被μODT直接测量的νRBC被明显低估了（νN≈0.27±0.03 mm / s，νH≈0.37±0.05 mm / s）。然而，所测得的νRBC增长（35.3％）与pc-μODT的νRBC增长（36.1％±±5.3％）相匹配。
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