Proof of concept for the simplified breakdown of cellulose by combining Pseudomonasputida strains with surface displayed thermophilic endocellulase exocellulase and β-glucosidase

摘要

BackgroundThe production and employment of cellulases still represents an economic bottleneck in the conversion of lignocellulosic biomass to biofuels and other biocommodities. This process could be simplified by displaying the necessary enzymes on a microbial cell surface. Such an approach, however, requires an appropriate host organism which on the one hand can withstand the rough environment coming along with lignocellulose hydrolysis, and on the other hand does not consume the generated glucose so that it remains available for subsequent fermentation steps.