Limonoids from seeds of Azadirachta indica A. Juss. and their cytotoxic activity

摘要

Four new limonoid-type nortriterpenoids, 1-detigloyl-1-O-methacryloylsalannin (>1), 28-deoxo-2,3-dihydronimbolide (>2), 12-acetoxy-3-O-acetyl-7-O-tigloylvilasinin (>3) and 12-acetoxy-3-O-acetyl-7-O-methacryloylvilasinin (>4), along with five known ones, were isolated from seeds of Azadirachta indica A. Juss. Their structures were elucidated by various spectroscopic methods, including UV, IR, MS, NMR, X-ray crystallography, quantum chemical calculation, as well as by comparison of their spectroscopic data with those reported. In the in vitro cytotoxic assay, >2 showed inhibitory activity against human breast cancer MDA-MB-231 cell line with IC50 value of 7.68±1.74 μmol/L, and >5 inhibited growth of human cervical cancer Hela cell line, melanoma A375 cell line and promyelocytic leukemia HL-60 cell line, with IC50 12.00±2.08, 17.44±2.11, and 13.95±5.74 μmol/L, respectively.