In vitro Cytotoxicity Effects of 197Au/PAMAMG4 and 198Au/PAMAMG4 Nanocomposites Against MCF7 and 4T1 Breast Cancer Cell Lines

摘要

>Purpose: Study on gold based therapeutic agents for cancer cells deracination has become under scrutiny in recent years owing to effective treatments are not available for rapidly progressive cancers. The aim of present study was to examine efficiency of radioactive ¹⁹⁸Au/PAMAMG4 and non-radioactive ¹⁹⁷Au/PAMAMG4 nancomposites against 4T1 and MCF7 breast cancer cell lines.>Methods: The PAMAMG4 dendrimer was treated with the gold anions and then, the mixture was chemically reduced by NaBH4. Prepared ¹⁹⁷Au/PAMAMG4 was bombarded by thermal neutrons in the Tehran Research Reactor to ¹⁹⁸Au/PAMAMG4 be produced. Prepared nanocomposites were characterized by means of FT-IR, 1H NMR, Zeta-potential measurements, TEM and EDX analyses. The radionuclidic purity of the ¹⁹⁸Au/PAMAMG4 solution was determined using purity germanium (HPGe) spectroscopy and its stability in the presence of human serum was studied. In vitro studies were carried out to compare toxicity of PAMAMG4, ¹⁹⁷Au/PAMAMG4 and ¹⁹⁸Au/PAMAMG4 towards 4T1 and MCF7 cancerous cells and C2C12 normal cell lines.>Results: Characterization results exhibited that invitro agents, ¹⁹⁷Au/PAMAMG4 and ¹⁹⁸Au/PAMAMG4, were synthesized successfully. Cells viability after 24 h, 48 h, and 72 h incubation, using MTT assay showed that the toxicity of ¹⁹⁸Au/PAMAMG4 is significantly superior in comparison with ¹⁹⁷Au/PAMAMG4 and PAMAMG4. Furthermore, the toxicity of ¹⁹⁸Au/PAMAMG4 was higher on cancerous cells especially in higher level of concentrations after 72 hours (P<0.05).>Conclusion: In the current study, the preparation of ¹⁹⁷Au/PAMAMG4 and ¹⁹⁸Au/PAMAMG4 is described and the cytotoxic properties of them against the MCF7, 4T1 cancerous cells and C2C12 normal cells were evaluated using MTT assay.