tRNA Modification and Genetic Code Variations in Animal Mitochondria

摘要

In animal mitochondria, six codons have been known as nonuniversal genetic codes, which vary in the course of animal evolution. They are UGA (termination codon in the universal genetic code changes to Trp codon in all animal mitochondria), AUA (Ile to Met in most metazoan mitochondria), AAA (Lys to Asn in echinoderm and some platyhelminth mitochondria), AGA/AGG (Arg to Ser in most invertebrate, Arg to Gly in tunicate, and Arg to termination in vertebrate mitochondria), and UAA (termination to Tyr in a planaria and a nematode mitochondria, but conclusive evidence is lacking in this case). We have elucidated that the anticodons of tRNAs deciphering these nonuniversal codons (tRNA^Trp for UGA, tRNA^Met for AUA, tRNA^Asn for AAA, and tRNA^Ser and tRNA^Gly for AGA/AGG) are all modified; tRNA^Trp has 5-carboxymethylaminomethyluridine or 5-taurinomethyluridine, tRNA^Met has 5-formylcytidine or 5-taurinomethyluridine, tRNA^Ser has 7-methylguanosine and tRNA^Gly has 5-taurinomethyluridine in their anticodon wobble position, and tRNA^Asn has pseudouridine in the anticodon second position. This review aims to clarify the structural relationship between these nonuniversal codons and the corresponding tRNA anticodons including modified nucleosides and to speculate on the possible mechanisms for explaining the evolutional changes of these nonuniversal codons in the course of animal evolution.