Interactive effect of dietary vitamin E and inorganic mercury on growth performance and bioaccumulation of mercury in juvenile olive flounder Paralichthys olivaceus treated with mercuric chloride

摘要

A 6-week feeding trial was carried out to evaluate the effects of dietary vitamin E (dl-α-tocopheryl acetate, TA) on growth and mercury (Hg) accumulation in juvenile olive flounder (Paralichthys olivaceus) treated with mercuric chloride (HgCl2). Vitamin E and HgCl2 were added to the semi-purified basal diet. Six semi-purified diets in a 2 × 3 factorial design were formulated to contain 2 levels of Hg (0 or 20 mg HgCl2/kg diet) and 3 levels of vitamin E (0, 100, or 200 mg TA/kg diet). Experimental fish (n = 360, 9.99 ± 0.15 g) were randomly allocated into 30-L tanks at a density of 20 fish per tank with 3 replicates in each treatment and were fed twice a day. At the end of the feeding trial, dietary Hg depressed the growth performances in terms of weight gain (WG), specific growth rate (SGR), feed efficiency (FE) and protein efficiency ratio (PER) in fish, while fish fed the diets supplemented with vitamin E showed significant growth improvement in both presence and absence of HgCl2 in the diets (P < 0.05). Survival rate was not affected in fish fed the experimental diets. Whole body compositions of fish such as lipid and moisture contents were influenced by dietary vitamin E supplementation. Total Hg contents of muscle, liver and kidney tissues were significantly reduced in fish fed diets supplemented with vitamin E (P < 0.05), while the two-way ANOVA showed that increasing Hg concentration has resulted in a reduction in vitamin E. Whole body fatty acids of fish like eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) contents were decreased by dietary Hg. However, supplementation of dietary vitamin E improved the α-linolenic acid (ALA) and EPA contents in fish. Our results suggest that dietary supplementation of vitamin E has potential effects on growth improvement and ameliorating inorganic Hg bioaccumulation in juvenile olive flounder.