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Characterization of a Family GH5 Xylanase with Activity on Neutral Oligosaccharides and Evaluation as a Pulp Bleaching Aid

机译:具有中性寡糖活性的家族GH5木聚糖酶的表征和纸浆漂白助剂的评价

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摘要

A new bacterial xylanase belonging to family 5 of glycosyl hydrolases was identified and characterized. The xylanase, Xyn5B from Bacillus sp. strain BP-7, was active on neutral, nonsubstituted xylooligosaccharides, showing a clear difference from other GH5 xylanases characterized to date that show a requirement for methyl-glucuronic acid side chains for catalysis. The enzyme was evaluated on Eucalyptus kraft pulp, showing its effectiveness as a bleaching aid.
机译:鉴定并鉴定了一种属于糖基水解酶家族5的新细菌木聚糖酶。木聚糖酶,来自芽孢杆菌属的Xyn5B。菌株BP-7对中性,未取代的木寡糖具有活性,显示出与迄今已表征的其他GH5木聚糖酶明显不同,这些酶显示需要甲基葡糖醛酸侧链来催化。在桉树牛皮纸浆上评估了该酶,显示了其作为漂白助剂的有效性。

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