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Occurrence of sep Insecticidal Toxin Complex Genes in Serratia spp. and Yersinia frederiksenii

机译:沙雷氏菌中sep杀虫毒素复合物基因的出现。和弗氏耶尔森氏菌

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摘要

Some strains of Serratia entomophila and S. proteamaculans cause amber disease of the grass grub Costelytra zealandica (Coleoptera: Scarabaeidae). Three genes required for virulence, sepABC, are located on a large plasmid, pADAP. Sequence analysis suggests that the sepABC gene cluster may be part of a horizontally mobile region. This study presents evidence for the putative mobility of the sep genes of pADAP. Southern blot analysis showed that orthologues of the sep genes reside on plasmids within S. entomophila, S. liquefaciens, S. proteamaculans, and a plasmid from Yersinia frederiksenii. Three plasmids hybridized to the pADAP sep virulence-associated region but not the pADAP replication and conjugation regions. Subsequent DNA sequence analysis of the Y. frederiksenii sep-like genes, designated tcYF1 and tcYF2, showed that they had 88% and 87% DNA identity to sepA and sepB, respectively. These results indicate that the sep genes are part of a discrete horizontally mobile region.
机译:某些粘质沙雷氏菌和proteamaculans菌株会引起草地gCostelytra zealandica(鞘翅目:Scarabaeidae)的琥珀色病。毒性所需的三个基因sepABC位于大质粒pADAP上。序列分析表明sepABC基因簇可能是水平移动区域的一部分。这项研究为pADAP的sep基因的假定迁移提供了证据。 Southern印迹分析表明,sep基因的直向同源物存在于嗜食链球菌,液化链球菌,proteamaculans链球菌和弗氏耶尔森氏菌的质粒内。三种质粒与pADAP sep毒力相关区域杂交,但未与pADAP复制和缀合区域杂交。随后对弗雷德里克氏酵母Sep-like基因的DNA序列分析,分别命名为tcYF1和tcYF2,表明它们分别与sepA和 sepB 具有88%和87%的DNA同一性。这些结果表明 sep 基因是一个离散的水平移动区域的一部分。

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