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New Bradyrhizobium japonicum Strains That Possess High Copy Numbers of the Repeated Sequence RSα

机译:具有重复序列RSα高拷贝数的新的日本根瘤菌

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摘要

In a survey of DNA fingerprints of indigenous Bradyrhizobium japonicum with the species-specific repeated sequences RSα and RSβ, 21 isolates from three field sites showed numerous RS-specific hybridization bands. The isolates were designated highly reiterated sequence-possessing (HRS) isolates, and their DNA hybridization profiles were easily distinguished from the normal patterns. Some HRS isolates from two field sites possessed extremely high numbers of RSα copies, ranging from 86 to 175 (average, 128), and showed shifts and duplications of nif- and hup-specific hybridization bands. The HRS isolates exhibited slower growth than normal isolates, although no difference in symbiotic properties was detected between the HRS and normal isolates. Nucleotide sequence analysis of 16S rRNA genes showed that HRS isolates were strains of B. japonicum. There was no difference in the spectra of serological and hydrogenase groupings of normal and HRS isolates. Some HRS isolates possessed a tandem repeat RSα dimer that is similar to the structure of (IS30)2, which was shown to cause a burst of transpositional rearrangements in Escherichia coli. The results suggest that HRS isolates are derived from normal isolates in individual fields by genome rearrangements that may be mediated by insertion sequences such as RSα.
机译:在对具有物种特异性重复序列RSα和RSβ的日本根瘤菌根瘤菌的DNA指纹进行的一项调查中,来自三个田地的21个分离株显示出许多RS特异性杂交带。这些分离株被指定为高度重复序列拥有(HRS)分离株,其DNA杂交谱很容易与正常模式区分开。来自两个田间地点的一些HRS分离株具有极高的RSα拷贝数,范围从86到175(平均128),并且显示了nif和hup特异性杂交带的移动和重复。尽管在HRS和正常分离株之间未检测到共生特性差异,但HRS分离株的生长速度比正常分离株慢。 16S rRNA基因的核苷酸序列分析表明,HRS分离株是日本芽孢杆菌的菌株。正常和HRS分离株的血清学和氢化酶分组的光谱没有差异。一些HRS分离株具有串联重复RSα二聚体,类似于(IS30)2的结构,该结构被证明会引起大肠杆菌中的转座重排。结果表明,HRS分离株是通过基因组重排从各个领域的正常分离株衍生而来的,而基因组重排可能是由插入序列(如RSα)介导的。

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