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Zwittermicin A-producing strains of Bacillus cereus from diverse soils.

机译:来自不同土壤的蜡状芽孢杆菌产Zwittermicin A菌株。

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摘要

Bacillus cereus UW85 produces a novel aminopolyol antibiotic, zwittermicin A, that contributes to the ability of UW85 to suppress damping-off of alfalfa caused by Phytophthora medicaginis. UW85 produces a second antibiotic, provisionally designated antibiotic B, which also contributes to suppression of damping-off but has not been structurally defined yet and is less potent than zwittermicin A. The purpose of this study was to isolate genetically diverse strains of B. cereus that produce zwittermicin A and suppress disease. We found that most isolates of B. cereus that were sensitive to phage P7 or inhibited the growth of Erwinia herbicola produced zwittermicin A; therefore, phage typing and E. herbicola inhibition provided indirect, but rapid screening tests for identification of zwittermicin A-producing isolates. We used these tests to screen a collection of 4,307 B. cereus and Bacillus thuringiensis isolates obtained from bacterial stock collections and from diverse soils collected in Honduras, Panama, Australia, The Netherlands, and the United States. A subset of the isolates screened by the P7 sensitivity and E. herbicola inhibition tests were assayed directly for production of zwittermicin A, leading to the identification of 57 isolates that produced zwittermicin A; 41 of these isolates also produced antibiotic B. Eight isolates produced antibiotic B but not zwittermicin A. The assay for phage P7 sensitivity was particularly useful because of its simplicity and rapidity and because 22 of the 23 P7-sensitive isolates tested produced zwittermicin A. However, not all zwittermicin A-producing isolates were sensitive to P7, and the more labor-intensive E. herbicola inhibition assay identified a larger proportion of the zwittermicin A producers.(ABSTRACT TRUNCATED AT 250 WORDS)
机译:蜡状芽孢杆菌UW85产生了一种新型的氨基多元醇抗生素,zwittermicin A,有助于UW85抑制由疫霉菌引起的苜蓿的衰减。 UW85产生第二种抗生素,临时指定为抗生素B,它也有助于抑制抑制作用,但尚未在结构上进行定义,且效力不如两性霉素A。这项研究的目的是分离蜡状芽孢杆菌的遗传多样性菌株。产生两性白蚁素A并抑制疾病。我们发现,大多数对噬菌体P7敏感或抑制草食欧文氏杆菌生长的蜡状芽孢杆菌分离株均产生了zwittermicinA。因此,噬菌体分型和除草大肠杆菌的抑制提供了间接的,但快速的筛选试验,用于鉴定产生兹威特霉素A的分离株。我们使用这些测试筛选了4307种蜡状芽孢杆菌和苏云金芽孢杆菌分离株,这些分离株是从细菌种群和洪都拉斯,巴拿马,澳大利亚,荷兰和美国的不同土壤中获得的。直接通过P7敏感性和草甘蓝抑制试验筛选的分离株的子集可检测出zwittermicin A的产生,从而鉴定出57株产生zwittermicin A的分离株。这些分离株中有41个也产生了抗生素B。8个分离株产生了抗生素B,但不产生两维白霉素A。噬菌体P7敏感性的测定特别有用,因为它的简便性和快速性,而且在测试的23种对P7敏感的分离物中,有22个产生了两维特霉素A。 ,并非所有生产zwittermicin A的分离株都对P7敏感,而劳动强度更高的草甘蓝抑制试验则确定了更大的zwittermicin A生产者比例。(摘要截断为250字)

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