Bacteria that are capable of degrading polycyclic aromatic hydrocarbons were enumerated by incorporating soil and water dilutions together with fine particles of phenanthrene, a polycyclic aromatic hydrocarbon, into an agarose overlayer and pouring the mixture over a mineral salts underlayer. The phenanthrene-degrading bacteria embedded in the overlayer were recognized by a halo of clearing in the opaque phenanthrene layer. Diesel fuel- or creosote-contaminated soil and water that were undergoing bioremediation contained 6 x 10(6) to 100 x 10(6) phenanthrene-degrading bacteria per g and ca. 5 x 10(5) phenanthrene-degrading bacteria per ml, respectively, whereas samples from untreated polluted sites contained substantially lower numbers. Unpolluted soil and water contained no detectable phenanthrene degraders (desert soil) or only very modest numbers of these organisms (garden soil, municipal reservoir water).
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机译：通过将土壤和水稀释液以及菲的细颗粒（多环芳烃）掺入琼脂糖上层，并将混合物倒在矿物盐下层上，列举出能够降解多环芳烃的细菌。包埋在覆盖层中的菲降解细菌通过不透明菲层中的透明光晕来识别。受到生物修复的受柴油燃料或杂酚油污染的土壤和水每g和ca含有6 x 10（6）至100 x 10（6）菲降解细菌。每毫升分别有5 x 10（5）个菲降解细菌，而未经处理的受污染部位的样品中细菌的数量要少得多。未污染的土壤和水中没有可检测到的菲降解物（沙漠土壤），或仅含有少量此类生物（花园土壤，市政水库水）。
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