Development and Comparison of In-house Line Probe Assay (LiPA) and SYBR Green Real-time PCR Regarding the Detection of Periodontal Pathogens

机译：关于牙周病原体检测的室内线探针测定法（LiPA）和SYBR Green实时PCR的开发和比较

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摘要

Background:Periodontal disease, which can become a chronic condition, is an inflammatory disease that upsets the soft and hard structures supporting the teeth. The aim of the present study was to design and develop an in-house Line Probe Assay (LiPA), to detect putative periodontitis-related bacterial pathogens, and compare it with SYBR Green Real-time PCR.

机译：背景：牙周疾病可以成为慢性疾病，是一种发炎性疾病，会破坏支撑牙齿的软硬结构。本研究的目的是设计和开发内部线探针检测（LiPA），以检测与牙周炎有关的细菌病原体，并将其与SYBR Green实时PCR进行比较。

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期刊名称 Avicenna Journal of Medical Biotechnology
作者
Mohammad Soleimani; Mohammad Reza Zolfaghari;
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作者单位

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年(卷),期 2019(11),1
年度 2019
页码 80–87
总页数 8
原文格式 PDF
正文语种
中图分类生物学;
关键词
Periodontitis Real-time PCR Sensitivity and specificity;

机译：牙周炎;实时荧光定量PCR;敏感性和特异性;

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1. Real-time RT-PCR assay for rapid and specific detection of classical swine fever virus: Comparison of SYBR Green and TaqMan MGB detection methods using novel MGB probes. [J] . Jamnikar-Ciglenecki U, Grom J, Toplak I, Journal of Virological Methods . 2008,第2期

机译：用于经典猪瘟病毒快速，特异性检测的实时RT-PCR分析：使用新型MGB探针对SYBR Green和TaqMan MGB检测方法的比较。
2. Molecular detection of human rhinoviruses in respiratory samples: a comparison of Taqman probe-, SYBR green I- and BOXTO-based real-time PCR assays [J] . Julien Dupouey, Laetitia Ninove, Vanessa Ferrier, Virology Journal . 2014,第1期

机译：呼吸道样本中人类鼻病毒的分子检测：基于Taqman探针，SYBR green I和BOXTO的实时PCR分析的比较
3. Development and validation of SYBR Green- and probe-based reverse-transcription real-time PCR assays for detection of the S and M segments of Schmallenberg virus [J] . Azkur Ahmet Kursat, van der Poel Wim H. M., Aksoy Emel, Journal of Veterinary Diagnostic Investigation . 2020,第5期

机译：基于SYBR绿色和探针的逆转录实时PCR测定的开发与验证，用于检测SCHMALLENBERG病毒的S和M分段
4. Development and primary application of the SYBR Green I RT-qPCR assay for the detection of the transmissible gastroenteritis virus (TGEV) S gene [C] . Gu Jiangping, Yue Xiuwei, Yuan Congli, 2011 4th International Conference on Biomedical Engineering and Informatics . 2011

机译：SYBR Green I RT-qPCR检测方法的开发和初步应用，用于检测可传播的胃肠炎病毒（TGEV）S基因
5. Development of a real-time PCR assay for detection and quantification of Escherichia coli O157:H7 in apple juice. [D] . DeTrana, Nancy Rabalais. 2007

机译：开发用于检测和定量苹果汁中大肠杆菌O157：H7的实时PCR检测方法。
6. Molecular detection of human rhinoviruses in respiratory samples: a comparison of Taqman probe- SYBR green I- and BOXTO-based real-time PCR assays [O] . Julien Dupouey, Laetitia Ninove, Vanessa Ferrier, 2014

机译：呼吸道样本中人类鼻病毒的分子检测：基于Taqman探针SYBR green I和BOXTO的实时PCR分析的比较
7. Molecular detection of human rhinoviruses in respiratory samples: a comparison of Taqman probe-, SYBR green I- and BOXTO-based real-time PCR assays [O] . Julien Dupouey, Laetitia Ninove, Vanessa Ferrier, 2014

机译：呼吸道样本中人类鼻病毒的分子检测：基于Taqman探针，SYBR green I和BOXTO的实时PCR分析的比较

Development and Comparison of In-house Line Probe Assay (LiPA) and SYBR Green Real-time PCR Regarding the Detection of Periodontal Pathogens

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